Ischemia / Reperfusion
Study Tissue Ischemia and Blood Reperfusion in Real Time
Obtain real-time dynamic measurements of oxygen saturation and vascular reperfusion in your disease model.
Tissue ischemia, as well as blood reperfusion in ischemic organs, can be investigated in vivo and in real-time with the Vevo F2 LAZR-X photoacoustic system.
The physiological and biochemical changes induced in the affected tissue or organ hold important clinical relevance in studies of disease and development of therapeutics.
Features include:
- Anatomy-specific oxygen saturation and total hemoglobin measurement
- Detection and quantification of biomarkers (eg. VEGFR2, VCAM)
- Assessing angiogenesis progression
- Quantifying perfusion kinetics
- Blood flow measurements
Example Study: Acute ischemia induced in mouse hindlimb with a tourniquet showing oxygen saturation during ischemia and post-reperfusion
Mouse hindlimb with tourniquet
Acute ischemia
Post-reperfusion
Use a Vevo System for:
- Femoral Artery Ligation models
- Peripheral Artery Disease assessment
- Ischemic Bowel Disease (Ischaemic Colitis)
- Myocardial infarct
- Stroke studies
- Wound healing and tissue injury
Gallery
Stem cells in the mouse hindlimb
3D rendered spectrally unmixed photoacoustic image of a stem-cell injected, ischemic (right) and control (left) mouse hindlimb. Oxyhemoglobin (red), deoxyhemoglobin (blue) and dye-labelled stem cells (green) are shown.
Ischemia/hypoxia stroke model
Plot of oxygen saturation (sO2) over time in the left (pink) and right (orange) cortical and subcortical hemisphere ROIs while restoring breathed oxygen to 100% from 7.5% post ischemia/hypoxia for stroke induction. Ultrasound (top) and photoacoustic (bottom) coronal images in the right panel show a frame of mouse brain post ischemia/hypoxia.
Ischemia/hypoxia stroke model
Ultrasound (left) and photoacoustic (right) coronal images of a mouse brain post ischemia/hypoxia. The red, white and blue color represents a parametric map of oxygen saturation. The right hemisphere does not fully recover sO2 levels post-reperfusion/normoxia that are shown in the left hemisphere, indicating a cerebral infarct, especially in cortical and subcortical areas.
Mouse hindlimb post-reperfusion
3D rendered power Doppler image of the mouse hindlimb showing blood flow. Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb, then relieved to allow reperfusion.
Mouse hindlimb post-reperfusion
3D rendered photoacoustic image of the mouse hindlimb showing a parametric map of oxygen saturation with red denoting higher sO2 values. Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb, then relieved to allow reperfusion. Higher sO2 is seen in the distal part of the limb post-reperfusion.
Mouse hindlimb during ischemia
3D rendered photoacoustic image of the mouse hindlimb showing a parametric map of oxygen saturation with red denoting higher sO2 values. Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb. Much lower sO2 is seen in the distal part of the limb during this acute ischemia.
Mouse hindlimb with tourniquet
3D rendered high-resolution ultrasound image of the mouse hindlimb showing a depression in the skin from a tourniquet made of tubing being used to restrict blood flow into the distal part of the limb.
Ischemia/hypoxia stroke model
3D rendered coronal ultrasound (greyscale) and photoacoustic (red) images of a mouse brain during ligation of the right common carotid artery. The red color represents higher oxygen saturation clearly showing lower sO2 in the right hemisphere.
Publications
Neurovascular hypoxia trajectories assessed by photoacoustic imaging in a murine model of cardiac arrest and resuscitation
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
, Neurovascular hypoxia trajectories assessed by photoacoustic imaging in a murine model of cardiac arrest and resuscitation
IEEE Trans Ultrason Ferroelectr Freq Control
, Monitoring Muscle Perfusion in Rodents During Short-Term Ischemia Using Power Doppler Ultrasound
Ultrasound in Medicine & Biology
, Comparable Impairment of Vascular Endothelial Function by a Wide Range of Electronic Nicotine Delivery Devices
Nicotine and Tobacco Research
, 3D-Printed Reinforcement Scaffolds with Targeted Biodegradation Properties for the Tissue Engineering of Articular Cartilage
Advanced Healthcare Materials
, Dental Pulp Mesenchymal Stem Cells Attenuate Limb Ischemia via Promoting Capillary Proliferation and Collateral Development in a Preclinical Model
Stem Cells International
, Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia
Cell Death and Disease
, Experimental Myocardial Infarction Elicits Time-Dependent Patterns of Vascular Hypoxia in Peripheral Organs and in the Brain
Frontiers in Cardiovascular Medicine
, Brain-targeted hypoxia-inducible factor stabilization reduces neonatal hypoxic-ischemic brain injury
Neurobiology of Disease
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