A heart–brain–kidney network controls adaptation to cardiac stress through tissue macrophage activation

Heart failure is a complex clinical syndrome characterized by insufficient cardiac function. In addition to abnormalities intrin to the heart, dysfunction of other organs and dysregulation of systemic factors greatly affect the development and consequen of heart failure. Here we show that the heart and kidneys function cooperatively in generating an adaptive response to cardiac pressure overload. In mice subjected to pressure overload in the heart, sympathetic nerve activation led to activation of renal collecting-duct (CD) epithelial cells. Cell–cell interactions among activated CD cells, tissue macrophages and endothelial cel within the kidney led to secretion of the cytokine CSF2, which in turn stimulated cardiac-resident Ly6Clo macrophages, whic are essential for the myocardial adaptive response to pressure overload. The renal response to cardiac pressure overload was disrupted by renal sympathetic denervation, adrenergic b2-receptor blockade or CD-cell-specific deficiency of the transcripti factor KLF5. Moreover, we identified amphiregulin as an essential cardioprotective mediator produced by cardiac Ly6Clo macrophages. Our results demonstrate a dynamic interplay between the heart, brain and kidneys that is necessary for adapta to cardiac stress, and they highlight the homeostatic functions of tissue macrophages and the sympathetic nervous system